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    Comparison of multilocus sequence typing (MLST) and repetitive sequence-based PCR (rep-PCR) fingerprinting for differentiation of Campylobacter jejuni isolated from broiler in Chiang Mai, Thailand (2012)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Patchanee, Prapas
    Chokboonmongkol, Chomporn
    Zessin, Karl-Hans
    Alter, Thomas (WE 8)
    Pornaem, Sarinya
    Chokesajjawatee, Nipa
    Forschungsprojekt
    FBI - ZOO: Untersuchungen zur Tenazität ausgewählter Campylobacter jejuni/coli- und Yersinia enterocolitica-Stämme in Lebensmittelmatrizen (TP 08)
    Quelle
    Journal of microbiology and biotechnology; 22(11) — S. 1467–1470
    ISSN: 1738-8872
    Sprache
    Englisch
    Verweise
    DOI: 10.4014/jmb.1112.12049
    Pubmed: 23124335
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    Tel.+49 30 838 62550 Fax.+49 30 838 46029
    email:lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    We compared rapid fingerprinting using repetitive sequence-based PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.