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    A multiplex ligation detection assay for the characterization of Salmonella enterica strains (2011)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Aarts, Henk J M
    Vos, Pieter
    Larsson, Jonas T
    van Hoek, Angela H A M
    Huehn, Stephan
    Weijers, Thijs
    Grønlund, Hugo Ahlm
    Malorny, Burkhard
    Quelle
    International Journal of Food Microbiology; 145(Suppl. 1) — S. 68–78
    ISSN: 0168-1605
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.ijfoodmicro.2010.10.010
    Pubmed: 21093086
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    Tel.+49 30 838 62550 Fax.+49 30 838 46029
    email:lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella 'test' strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.