Fachbereich Veterinärmedizin



    Fluorimetric characterisation of metabolic activity of ex vivo perfused pig hearts (2007)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Pfeifer, Lutz
    Gruenwald, Inga
    Welker, Alexander
    Stahn, Romana M
    Stein, Karsten
    Rex, André
    Biomedizinische Technik. Biomedical engineering; 52(2) — S. 193–199
    ISSN: 0013-5585
    Pubmed: 17408379
    Institut für Pharmakologie und Toxikologie

    Koserstr. 20
    14195 Berlin
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    Abstract / Zusammenfassung

    Autofluorescence of tissues and organs is an indicator of the physiological state of cells. The aim of the study was to investigate whether fluorimetric determination of the redox state of the ex vivo perfused pig heart can provide fast online detection of progressive changes in heart muscle tissue. Measurements on six organs perfused in a four-chamber working heart model were performed using a spectroscopic method exploiting the specific and different fluorescence lifetimes of intrinsic fluorophores such as NADH and flavins and providing a means of internal signal referencing. It was shown that the redox potential of heart muscle tissue can be assessed by fluorescence measurement. In the steady-state phase of the beating heart, spectroscopic measurements revealed a change in redox state from an initial constant level to a continuous decrease, accompanied by a decrease in heart performance and indications of changes in electrolyte equilibrium (K(+) concentration). At the same time, troponin I levels in the perfusate increased. The results indicate that fluorimetric determination of heart muscle metabolic activity yields reliable information about the functional status of the ex vivo heart and may be advantageous for the optimisation of ex vivo organ models.