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    Empfindlichkeitsprüfung von Bakterien gegenüber antimikrobiellen Wirkstoffen in der Routinediagnostik:
    Vorschlag für ein Layout für Mikrotiterplatten zur Testung von Bakterien von Hunden und Katzen (2008)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Werckenthin, Christiane
    Luhofer, Gabriele
    Böttner, Alexander
    Gangl, Armin
    Goossens, Luc
    Hafez, H Mohamed
    Hartmann, Katrin
    Kaske, Martin
    Kehrenberg, Corinna
    Kietzmann, Manfred
    Klarmann, Dieter
    Klein, Günter
    Krabisch, Peter
    Kühn, Tilman
    Richter, Angelika
    Schulz, Bianka
    Schwarz, Stefan
    Sigge, Claudia
    Traeder, Wolfgang
    Waldmann, Karl-Heinz
    Wallmann, Jürgen
    Quelle
    Berliner und Münchener tierärztliche Wochenschrift; 121(1/2) — S. 19–26
    ISSN: 0005-9366
    Verweise
    Pubmed: 18277776
    Kontakt
    Institut für Pharmakologie und Toxikologie

    Koserstr. 20
    14195 Berlin
    Tel.+49 30 838 53221 Fax.+49 30 838 53112
    email:pharmakologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The determination of minimum inhibitory concentrations by broth microdilution is recommended as method of choice for susceptibility testing of veterinary bacterial pathogens. Accordingly, broth microdilution is used in veterinary routine diagnostic laboratories at a progressive rate. To reduce the costs of susceptibility testing, it is reasonable to develop widely accepted uniform microtitre plate layouts that are produced in large quantities. Such microtitre plate layouts have already been developed and published for the susceptibility testing of pathogens from food-producing animals. However, a microtitre plate layout, especially designed for the testing of bacteria from dogs and cats, should be available, too. The choice of the antimicrobial agents or combinations of antimicrobial agents to be included in a suitable layout should be based on the following criteria: (1) the approval and availability of an antimicrobial agent or combination of agents, (2) known cross-resistances, and (3) availability of approved clinical breakpoints. The latter point is of particular importance for the choice of the numbers of concentrations per antimicrobial agent tested and the range of test concentrations. Taking into account these aspects, a science-based layout proposal for microtitre plates, which are suitable for routine testing of bacteria from dogs and cats, is presented and discussed.