Fachbereich Veterinärmedizin



    Neurodegeneration in newborn rats following propofol and sevoflurane anesthesia (2009)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Bercker, Sven
    Bert, Bettina
    Bittigau, Petra
    Felderhoff-Müser, Ursula
    Bührer, Christoph
    Ikonomidou, Chrysanthy
    Weise, Mirjam
    Kaisers, Udo X
    Kerner, Thoralf
    Neurotoxicity Research; 16(2) — S. 140–147
    ISSN: 1029-8428
    DOI: 10.1007/s12640-009-9063-8
    Pubmed: 19526290
    Institut für Pharmakologie und Toxikologie

    Koserstr. 20
    14195 Berlin
    Tel.+49 30 838 53221 Fax.+49 30 838 53112

    Abstract / Zusammenfassung

    Propofol and sevoflurane are commonly used drugs in pediatric anesthesia. Exposure of newborn rats to a variety of anesthetics has been shown to induce apoptotic neurodegeneration in the developing brain. Newborn Wistar rats were treated with repeated intraperitoneal injections of propofol or sevoflurane inhalation and compared to controls. Brains were examined histopathologically using the De Olmos cupric silver staining. Additionally, a summation score of the density of apoptotic cells was calculated for every brain. Spatial memory learning was assessed by the Morris Water Maze (MWM) test and the hole board test, performed in 7 weeks old animals who underwent the same anesthetic procedure. Brains of propofol-treated animals showed a significant higher neurodegenerative summation score (24,345) when compared to controls (15,872) and to sevoflurane-treated animals (18,870). Treated animals also demonstrated persistent learning deficits in the hole board test, whereas the MWM test revealed no differences between both groups. Among other substances acting via GABAA agonism and/or NMDA antagonism propofol induced neurodegeneration in newborn rat brains whereas a sevoflurane based anesthesia did not. The significance of these results for clinical anesthesia has not been completely elucidated. Future studies have to focus on the detection of safe anesthetic strategies for the developing brain.