Publikationsdatenbank

Plasmid-mediated quinolone resistance genes in fecal bacteria from rooks commonly wintering throughout Europe (2012)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Literak, Ivan

Micudova, Maria

Tausova, Dagmar

Cizek, Alois

Dolejska, Monika

Papousek, Ivo

Prochazka, Jakub

Vojtech, Jiri

Borleis, Frank

Guardone, Lisa

Guenther, Sebastian

Hordowski, Jozef

Lejas, Cyrille

Meissner, Wlodzimierz

Marcos, Benito Fuertes

Tucakov, Marko

Quelle

Microbial drug resistance : MDR ; mechanisms, epidemiology, and disease

Bandzählung: 18

Heftzählung: 6

Seiten: 567 – 573

ISSN: 1076-6294

Sprache

Englisch

Verweise

DOI: 10.1089/mdr.2012.0075

Pubmed: 22731858

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

This study concerned the occurrence of fecal bacteria with plasmid-mediated quinolone resistance (PMQR) genes in rooks (Corvus frugilegus, medium-sized corvid birds) wintering in continental Europe during winter 2010/2011. Samples of fresh rook feces were taken by cotton swabs at nine roosting places in eight European countries. Samples were transported to one laboratory and placed in buffered peptone water (BPW). The samples from BPW were enriched and subcultivated onto MacConkey agar (MCA) supplemented with ciprofloxacin (0.06 mg/L) to isolate fluoroquinolone-resistant bacteria. DNA was isolated from smears of bacterial colonies growing on MCA and tested by PCR for PMQR genes aac(6')-Ib, qepA, qnrA, qnrB, qnrC, qnrD, qnrS, and oqxAB. All the PCR products were further analyzed by sequencing. Ciprofloxacin-resistant bacteria were isolated from 37% (392 positive/1,073 examined) of samples. Frequencies of samples with ciprofloxacin-resistant isolates ranged significantly from 3% to 92% in different countries. The qnrS1 gene was found in 154 samples and qnrS2 in 2 samples. The gene aac(6')-Ib-cr was found in 16 samples. Thirteen samples were positive for qnrB genes in variants qnrB6 (one sample), qnrB18 (one), qnrB19 (one), qnrB29 (one), and qnrB49 (new variant) (one). Both the qnrD and oqxAB genes were detected in six samples. The genes qnrA, qnrC, and qepA were not found. Wintering omnivorous rooks in Europe were commonly colonized by bacteria supposedly Enterobacteriaceae with PMQR genes. Rooks may disseminate these epidemiologically important bacteria over long distances and pose a risk for environmental contamination.