Fachbereich Veterinärmedizin



    Rat hepatitis E virus:
    geographical clustering within Germany and serological detection in wild Norway rats (Rattus norvegicus) (2012)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Johne, Reimar
    Dremsek, Paul
    Kindler, Eveline
    Schielke, Anika
    Plenge-Bönig, Anita
    Gregersen, Henrike
    Wessels, Ute
    Schmidt, Katja
    Rietschel, Wolfram
    Groschup, Martin H
    Guenther, Sebastian
    Heckel, Gerald
    Ulrich, Rainer G
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases; 12(5) — S. 947–956
    ISSN: 1567-1348
    DOI: 10.1016/j.meegid.2012.02.021
    Pubmed: 22554648
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843

    Abstract / Zusammenfassung

    Zoonotic hepatitis E virus (HEV) infection in industrialised countries is thought to be caused by transmission from wild boar, domestic pig and deer as reservoir hosts. The detection of HEV-specific antibodies in rats and other rodents has suggested that these animals may represent an additional source for HEV transmission to human. Recently, a novel HEV (ratHEV) was detected in Norway rats from Hamburg, Germany, showing the typical genome organisation but a high nucleotide and amino acid sequence divergence to other mammalian and to avian HEV strains. Here we describe the multiple detection of ratHEV RNA and HEV-specific antibodies in Norway rats from additional cities in north-east and south-west Germany. The complete genome analysis of two novel strains from Berlin and Stuttgart confirmed the association of ratHEV to Norway rats. The present data indicated a continuing existence of this virus in the rat populations from Berlin and Hamburg. The phylogenetic analysis of a short segment of the open reading frame 1 confirmed a geographical clustering of the corresponding sequences. Serological investigations using recombinant ratHEV and genotype 3 capsid protein derivatives demonstrated antigenic differences which might be caused by the high amino acid sequence divergence in the immunodominant region. The high amount of animals showing exclusively ratHEV RNA or anti-ratHEV antibodies suggested a non-persistent infection in the Norway rat. Future studies have to prove the transmission routes of the virus in rat populations and its zoonotic potential. The recombinant ratHEV antigen generated here will allow future seroepidemiological studies to differentiate ratHEV and genotype 3 infections in humans and animals.