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Seeing the fact that farm managers in Germany feed anionic salts to transition cows once daily, this study set out to evaluate whether the effects on the acid-base status (ABS) and calcium excretion in urine would persist throughout the entire day beyond this feeding practice. Eleven non-lactating, non-pregnant, Holstein-Friesian-cows with a rumen fistula were administered 2Eq of calcium chloride (CaCl(2)/five cows) or calcium sulfate (CaSO(4)/six cows) once daily for a period of 1 week. At day 7, blood and urine samples were taken every 4h starting at 06:00 a.m. before feeding the anionic salts, and then ending at the same time the next day. Feeding anionic salts to the cows induced metabolic acidosis in both of the groups. The changes tended to be greater in CaCl(2)-cows. After 12h, the acidosis lessened and the initial values were reached after 24h. The CaCl(2)-cows, however, still showed signs of compensated metabolic acidosis. The results of the present study showed that feeding anionic salts once daily confined the risk of an interrupted effect of the anionic salts on the acid-base status as well as calcium metabolism after 12h.