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Four meat turkey and one turkey breeding flocks were surveyed for antibodies against reticuloendotheliosis virus (REV) at different intervals using commercial enzyme-linked immunosorbent assays (ELISAs). In addition, serum samples collected from 18 flocks at different ages were also tested for antibodies against REV. No antibodies were detected in any of the four meat turkey flocks that were surveyed. In the breeder flock, 20% of tested samples from 1-day-old poults were positive. Between the fourth and 12th weeks, all samples that were tested yielded negative results. At 16 weeks of age, 15% of samples yielded a positive reaction, but antibodies could not be detected 4 weeks later. Examination of serum samples from 18 different flocks at various ages revealed that antibodies could be detected in five flocks. The percentage of positive sera per flock ranged between 10 and 40%.