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    The use of PCR combined with restriction enzyme analysis to characterize fowl adenovirus field isolated from Northern Indian (2002)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Singh, A.
    Oberoi, M. S.
    Grewal, G. S.
    Hafez, H. M.
    Hess, M.
    Quelle
    Veterinary Research Communications; 26 — S. 577–585
    ISSN: 0165-7380
    Sprache
    Englisch
    Verweise
    Pubmed: 12416873
    Kontakt
    Institut für Geflügelkrankheiten

    Königsweg 63
    14163 Berlin
    +49 30 838 62676
    gefluegelkrankheiten@vetmed.fu-berlin

    Abstract / Zusammenfassung

    Ten fowl adenoviruses (FAVs), isolated from suspected cases of inclusion body hepatitis (IBH) in quails and broilers, were characterized by a hexon-based polymerase chain reaction (PCR) combined with restriction enzyme analysis (REA) of the amplified DNA fragments. All the isolates could be detected using H1/H2 and H3/H4 primer sets. Amplification of DNA with H1/H2 and H3/H4 primer sets resulted in fragments of approximately 1219 bp and 1319 bp, respectively. HaeII digestion of the H1/ H2 PCR products and HpaII digestion of the H3/H4 PCR products characterized all the isolates in FAV groups, known from genomic typing using the whole DNA. For some of the isolates, neutralization tests were used to confirm these results. The results revealed that, as well as FAV serotype 1, which is the sole member of DNA group A, FAVs of DNA group E are also associated with IBH in poultry in northern India. The FAV specific PCR combined with REA was found to be very useful in investigating the epidemiological situation in the field. It was even possible to define mixed infections with more than one FAV.