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    A fiber gene-based polymerase chain reaction for specific detection of pigeon adenovirus (2002)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Raue, R.
    Hafez, H. M.
    Hess, M.
    Quelle
    Avian pathology : journal of the W.V.P.A; 31 — S. 95–99
    ISSN: 0307-9457
    Sprache
    Englisch
    Verweise
    Pubmed: 12425796
    Kontakt
    Institut für Geflügelkrankheiten

    Königsweg 63
    14163 Berlin
    Tel.+49 30 838 62676 Fax.+49 30 838 62690
    email:gefluegelkrankheiten@vetmed.fu-berlin

    Abstract / Zusammenfassung

    A polymerase chain reaction (PCR) was developed to detect a recently described pigeon adenovirus (PiAV). Primers located in the fiber gene of PiAV amplified a PCR fragment solely from PiAV DNA, whereas all 12 serotypes of fowl adenoviruses (FAV1 to FAV12), some of them able to infect pigeons, did not react. A PCR fragment of 967 base pairs was amplified from three different isolates serologically typed as PiAV and from some pigeon liver samples showing morphological and histological signs of an adenovirus infection. Those samples did not react with a published primer pair (H3/H4) able to detect FAV, demonstrating the specificity of both PCRs to react exclusively with the respective pathogen, PiAV or FAV. The presented PCR is a suitable diagnostic tool to gain further insight into the epidemiology of PiAV infections in pigeons.