Publikationsdatenbank

A Vital Role of Tubulin-Tyrosine-Ligase for Neuronal Organization (2005)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Erck, Christian

Peris, Leticia

Andrieux, Annie

Meissirel, Claire

Gruber, Achim D

Vernet, Muriel

Schweitzer, Annie

Saoudi, Yasmina

Pointu, Hervé

Bosc, Christophe

Salin, Paul A

Job, Didier

Wehland, Juergen

Quelle

Proceedings of the National Academy of Sciences of the United States of America

Bandzählung: 102

Heftzählung: 22

Seiten: 7853 – 7858

ISSN: 1091-6490

Sprache

Englisch

Verweise

Pubmed: 15899979

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Tubulin is subject to a special cycle of detyrosination/tyrosination in which the C-terminal tyrosine of alpha-tubulin is cyclically removed by a carboxypeptidase and readded by a tubulin-tyrosine-ligase (TTL). This tyrosination cycle is conserved in evolution, yet its physiological importance is unknown. Here, we find that TTL suppression in mice causes perinatal death. A minor pool of tyrosinated (Tyr-)tubulin persists in TTL null tissues, being present mainly in dividing TTL null cells where it originates from tubulin synthesis, but it is lacking in postmitotic TTL null cells such as neurons, which is apparently deleterious because early death in TTL null mice is, at least in part, accounted for by a disorganization of neuronal networks, including a disruption of the cortico-thalamic loop. Correlatively, cultured TTL null neurons display morphogenetic anomalies including an accelerated and erratic time course of neurite outgrowth and a premature axonal differentiation. These anomalies may involve a mislocalization of CLIP170, which we find lacking in neurite extensions and growth cones of TTL null neurons. Our results demonstrate a vital role of TTL for neuronal organization and suggest a requirement of Tyr-tubulin for proper control of neurite extensions.