Publikationsdatenbank

Selection of reference genes for quantitative real-time PCR analysis in canine mammary tumors using the GeNorm algorithm (2006)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Etschmann, B

Wilcken, B

Stoevesand, K

von der Schulenburg, A

Sterner-Kock, A

Quelle

Veterinary pathology : an internat. journal of natural and experimental disease in animals

Bandzählung: 43

Heftzählung: 6

Seiten: 934 – 942

ISSN: 0300-9858

Sprache

Englisch

Verweise

Pubmed: 17099150

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Eleven reference genes (18s ribosomal ribonucleic acid [RNA], 28s ribosomal RNA, ubiquitin, beta-actin, glycerine aldehyde dehydrogenase, ATP-synthase subunit 5B, hydroxymethyl-bilane synthase, hypoxanthine-phosphoribosyl transferase, ribosomal protein L32, tryptophan 5-monooxygenase activation protein (zeta polypeptide), and TATA-Box binding protein) were analyzed in use as references for gene expression profiling experiments using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in canine mammary tumors. The transcription level of the candidates was measured in 22 histologically characterized excised tumor specimens from mammary gland tissue and 22 samples of non-neoplastic mammary tissue samples from the same individuals. Results were used to rank candidate reference genes using the GeNorm tool. It was determined that in samples of canine mammary gland tissue, a combination of hypoxanthine-phosphoribosyl transferase, ATP-synthase subunit 5B, ribosomal protein L32 and ubiquitin yields stable reference gene expression levels, whereas the use of glycerin aldehyde dehydrogenase or ribosomal RNA is unsuitable for normalization of qRT-PCR results in this tissue type.