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    Subtyping of new Brazilian avian metapneumovirus isolates from chickens and turkeys by reverse transcriptase-nested-polymerase chain reaction (2005)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    D'Arce, R C
    Cosig, L T
    Almeida, R S
    Trevisol, I M
    Monteiro, M C
    Rossini, L I
    Fabio J, D I
    Hafez, H M (WE 15)
    Arns, C. W.
    Quelle
    Avian pathology : journal of the W.V.P.A; 34 — S. 133–136
    ISSN: 0307-9457
    Sprache
    Englisch
    Verweise
    Pubmed: 16191694
    Kontakt
    Institut für Geflügelkrankheiten

    Königsweg 63
    14163 Berlin
    +49 30 838 62676
    gefluegelkrankheiten@vetmed.fu-berlin

    Abstract / Zusammenfassung

    The aim of this study was to improve a reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) able to differentiate avian pneumovirus (APV) subtypes A and B, and to characterize new Brazilian isolates. Representative APV strains and clinical field samples from chickens and turkey flocks were amplified in the chicken embryo-related cell line. Viral RNA was extracted from harvested cells, and submitted to cDNA synthesis. The primers utilized for RT-PCR were compatible with the G gene of both the A and B subtypes of APV, while the nested primers were subtype specific. This approach showed that three new APVs from chickens and one from turkeys were subtype A, confirmed by sequencing. This is the first report of APV isolation from turkeys in Brazil. Four other APVs were detected and classified as subtype A by RT-nested-PCR. These optimized techniques could be useful for differentiation of APV subtypes A and B, proving to be a valuable molecular epidemiological tool.