Publikationsdatenbank

The effect of short chain fatty acids on calcium flux rates across isolated rumen epithelium of hay-fed and concentrate-fed sheep (2003)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Uppal, S K

Wolf, K

Martens, H

Quelle

Journal of animal physiology and animal nutrition : official journal of the European Society of Veterinary and Comparative Nutrition and the American Academy of Veterinary Nutrition

Bandzählung: 87

Heftzählung: 1/2

Seiten: 12 – 20

ISSN: 0931-2439

Sprache

Englisch

Verweise

Pubmed: 14511145

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The present in vitro experiment was conducted to study the effect of two concentrations of short chain fatty acids [SCFA: 0 (control), 40 and 100 mmol/l in the buffer solution on mucosal side] on calcium ion (Ca2+) transport across the isolated rumen epithelium of two groups of sheep. One group was subjected to hay ad libitum, whereas the other to concentrate feed (800 g per day in equal portion at 7.00 and 15.00 hours and hay ad lib). The conventional Ussing chamber method was used for measuring the Ca2+ transport rates (45Ca), short-circuit current (Isc) and tissue conductance (GT) of isolated rumen epithelium. The SCFA significantly increased Isc of the epithelia of concentrate-fed sheep. In both hay- and concentrate-fed animals, 45Ca flux rates showed an almost linear increase in net flux rate () with rising concentrations of SCFA, as a result of a combined effect of a large increase in mucosal-to-serosal flux rates () and an almost linear, but small, decrease in serosal-to-mucosal flux rate (). In concentrate-fed sheep and were significantly higher in tissues incubated with SCFA compared with hay-fed animals. The well-known adaptable morphological and functional changes in the rumen epithelium attributable to concentrate feeding obviously include Ca2+ transport; such feeding therefore may be considered as a possible prophylactic measure in the prevention of milk fever.