Publikationsdatenbank

Quantitative Expression Analyses of Candidates for Alternative Anion Conductance in Cystic Fibrosis Mouse Models (2010)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Braun, Josephine

Mundhenk, Lars

Range, Friederike

Gruber, Achim D

Quelle

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society

Bandzählung: 9

Heftzählung: 5

Seiten: 351 – 364

ISSN: 1569-1993

Sprache

Englisch

Verweise

DOI: 10.1016/j.jcf.2010.06.003

Pubmed: 20624691

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Diversity of cystic fibrosis (CF) phenotype in patients with the same CFTR-mutation raised the hypothesis that other factors modulate the phenotype including "alternative" calcium-activated anion currents (CaCC). This study compares the mRNA expression levels of candidate CaCC mediators in CF mouse models with wild type controls.

mBEST1, mBEST2, mCLC-3B, mCLC-4, mTTYH3, mTMEM16A, mTMEM16F, mTMEM16K, mCLCA1 to -6 and SLC26A9 mRNA were quantified in CF-relevant tissues in cftr(tm1Cam) and cftr(TgH(neoim)Hgu) mice and controls using real-time RT-qPCR.

No consistent differences were observed except for mTTYH3 which was significantly down-regulated throughout the intestinal tract of cftr(tm1Cam) mice.

Down-regulation of mTTYH3 may point towards its involvement in the complex CF pathology. However, the markedly reduced expression argues against a direct compensatory action as an alternative anion conductance. If any of the other candidates plays a role as modulator, factors other than transcriptional regulation and mRNA stability may be involved.