Publikationsdatenbank

Murine mCLCA5 is Expressed in Granular Layer Keratinocytes of Stratified Epithelia (2010)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Braun, Josephine

Bothe, Melanie K

Mundhenk, Lars

Beck, Carol L

Gruber, Achim D

Quelle

Histochemistry and cell biology

Bandzählung: 133

Heftzählung: 3

Seiten: 285 – 299

ISSN: 0948-6143

Sprache

Englisch

Verweise

DOI: 10.1007/s00418-009-0667-0

Pubmed: 20012443

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

CLCA proteins represent a large family of proteins widely expressed in mammalian tissues with a unique expression pattern for each family member analyzed so far. However, their functions in normal and diseased tissues are poorly understood. Here, we present the cellular expression pattern of mCLCA5 in murine tissues using immunohistochemistry, confocal laser scanning microscopy and immune electron microscopy with specific antibodies and RT-qPCR following laser-capture microdissection. The mCLCA5 protein was localized to granular layer keratinocytes of virtually all stratified squamous epithelia of the body. Biochemical protein characterizations revealed that the amino-terminal cleavage product is fully secreted by the cell, while the carboxy-terminal cleavage product remains associated with the cell. The results imply that mCLCA5 may play a role in maturation and keratinization of squamous epithelial cells.