Fachbereich Veterinärmedizin


Service-Navigation

    Publikationsdatenbank

    Efficient and cost-effective extraction of genomic DNA from formalin-fixed and paraffin-embedded tissues (2011)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Weiss, A Th A
    Delcour, N M
    Meyer, A
    Klopfleisch, R
    Quelle
    Veterinary Pathology; 48(4) — S. 834–838
    ISSN: 0300-9858
    Sprache
    Englisch
    Verweise
    DOI: 10.1177/0300985810380399
    Pubmed: 20817894
    Kontakt
    Institut für Tierpathologie

    Robert-von-Ostertag-Str. 15
    Gebäude 12
    14163 Berlin
    +49 30 838 62450

    Abstract / Zusammenfassung

    Diagnostic and investigative molecular pathology frequently has to resort to extraction of DNA from formalin-fixed and paraffin-embedded tissue samples. Although many different protocols are reported for this type of material, extraction of sufficient amounts of intact DNA is still challenging. Here, the authors report a reproducible, simple, cost-effective, and efficient protocol that yields up to 140 μg of DNA from approximately 10 to 15 mg of formalin-fixed and paraffin-embedded tissue samples and compare it to available protocols. The protocol allows stable amplification of DNA fragments up to 600 bp in length in a wide variety of tissues.