Publikationsdatenbank

Efficient and cost-effective extraction of genomic DNA from formalin-fixed and paraffin-embedded tissues (2011)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Weiss, A Th A

Delcour, N M

Meyer, A

Klopfleisch, R

Quelle

Veterinary pathology : an internat. journal of natural and experimental disease in animals

Bandzählung: 48

Heftzählung: 4

Seiten: 834 – 838

ISSN: 0300-9858

Sprache

Englisch

Verweise

DOI: 10.1177/0300985810380399

Pubmed: 20817894

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Diagnostic and investigative molecular pathology frequently has to resort to extraction of DNA from formalin-fixed and paraffin-embedded tissue samples. Although many different protocols are reported for this type of material, extraction of sufficient amounts of intact DNA is still challenging. Here, the authors report a reproducible, simple, cost-effective, and efficient protocol that yields up to 140 μg of DNA from approximately 10 to 15 mg of formalin-fixed and paraffin-embedded tissue samples and compare it to available protocols. The protocol allows stable amplification of DNA fragments up to 600 bp in length in a wide variety of tissues.