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    Fungal Species Identification from Avian Lung Specimens by Single Hypha Laser Microdissection and PCR Product sequencing (2011)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Olias, Philipp
    Jacobsen, Ilse D
    Gruber, Achim D
    Quelle
    Medical mycology : official publication of the International Society for Human and Animal Mycology; 49(1) — S. 56–61
    ISSN: 1369-3786
    Sprache
    Englisch
    Verweise
    DOI: 10.3109/13693786.2010.497172
    Pubmed: 20608780
    Kontakt
    Institut für Tierpathologie

    Robert-von-Ostertag-Str. 15
    Gebäude 12
    14163 Berlin
    +49 30 838 62450

    Abstract / Zusammenfassung

    Accurate species diagnosis in cases of fungal pneumonia may be hampered by environmental contamination and colonization resulting in false-positive results. Our novel approach for fungal species diagnostics combines fluorescent staining of mounted cryosections with the optical brightener Blankophor, laser capture microdissection and PCR amplification with subsequent sequencing of the first internal transcribed spacer region (ITS-1). Using clinical specimens from infected birds, we show that the procedure is suitable for species identification from single hyphae of intralesional filamentous fungi. Our data also suggest that multiple Aspergillus fumigatus strain infections may occur frequently in pulmonary aspergillosis of birds.