Publikationsdatenbank

Fungal Species Identification from Avian Lung Specimens by Single Hypha Laser Microdissection and PCR Product sequencing (2011)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Olias, Philipp

Jacobsen, Ilse D

Gruber, Achim D

Quelle

Medical mycology : official publication of the International Society for Human and Animal Mycology

Bandzählung: 49

Heftzählung: 1

Seiten: 56 – 61

ISSN: 1369-3786

Sprache

Englisch

Verweise

DOI: 10.3109/13693786.2010.497172

Pubmed: 20608780

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Accurate species diagnosis in cases of fungal pneumonia may be hampered by environmental contamination and colonization resulting in false-positive results. Our novel approach for fungal species diagnostics combines fluorescent staining of mounted cryosections with the optical brightener Blankophor, laser capture microdissection and PCR amplification with subsequent sequencing of the first internal transcribed spacer region (ITS-1). Using clinical specimens from infected birds, we show that the procedure is suitable for species identification from single hyphae of intralesional filamentous fungi. Our data also suggest that multiple Aspergillus fumigatus strain infections may occur frequently in pulmonary aspergillosis of birds.