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    Schmallenberg virus challenge models in cattle:
    infectious serum or culture-grown virus? (2012)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Wernike, Kerstin
    Eschbaumer, Michael
    Breithaupt, Angele (WE 12)
    Hoffmann, Bernd
    Beer, Martin
    Quelle
    Veterinary Research; 43(1) — S. 84
    ISSN: 0928-4249
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://edocs.fu-berlin.de/docs/receive/FUDOCS_document_000000019620
    DOI: 10.1186/1297-9716-43-84
    Pubmed: 23231006
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    Gebäude 12
    14163 Berlin
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    Abstract / Zusammenfassung

    Schmallenberg virus (SBV), discovered in Europe in 2011, causes mild transient disease in adult ruminants, but fetal infection can lead to severe malformation in cattle, sheep and goats.To elucidate the pathogenesis of this novel orthobunyavirus, considerable efforts are required. A reliable and standardized infection model is essential for in vivo studies. In the present study, two groups of four cattle were inoculated with either serum passaged in cattle only or cell culture-grown virus. The replication of culture-grown SBV in cattle was reduced compared to virus inoculated via infectious serum. In a second experiment, the infectious serum was titrated in calves; the tested batch contained 102.83 infectious doses per mL. Hence, serum-borne virus that was only passaged in the natural host is a suitable option for a standardized SBV infection model.