Fachbereich Veterinärmedizin



    The Porcine Chloride Channel Calcium-activated Family Member pCLCA4a Mirrors Lung Expression of the Human hCLCA4 (2012)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Plog, Stephanie
    Grötzsch, Tanja
    Klymiuk, Nikolai
    Kobalz, Ursula
    Gruber, Achim D
    Mundhenk, Lars
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society; 60(1) — S. 45–56
    ISSN: 0022-1554
    DOI: 10.1369/0022155411426455
    Pubmed: 22205680
    Institut für Tierpathologie

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    Abstract / Zusammenfassung

    Pig models of cystic fibrosis (CF) have recently been established that are expected to mimic the human disease closer than mouse models do. The human CLCA (originally named chloride channels, calcium-activated) member hCLCA4 is considered a potential modifier of disease severity in CF, but its murine ortholog, mCLCA6, is not expressed in the mouse lung. Here, we have characterized the genomic structure, protein processing, and tissue expression patterns of the porcine ortholog to hCLCA4, pCLCA4a. The genomic structure and cellular protein processing of pCLCA4a were found to closely mirror those of hCLCA4 and mCLCA6. Similar to human lung, pCLCA4a mRNA was strongly expressed in porcine lungs, and the pCLCA4a protein was immunohistochemically detected on the apical membranes of tracheal and bronchial epithelial cells. This stands in sharp contrast to mouse mCLCA6, which has been detected exclusively in intestinal epithelia but not the murine lung. The results may add to the understanding of species-specific differences in the CF phenotype and support the notion that the CF pig model may be more suitable than murine models to study the role of hCLCA4.