Publikationsdatenbank

Synthesis of Porcine pCLCA2 Protein during Late Differentiation of Keratinocytes of Epidermis and Hair Follicle inner Root Sheath (2012)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Plog, Stephanie

Mundhenk, Lars

Langbein, Lutz

Gruber, Achim D

Quelle

Cell and tissue research

Bandzählung: 350

Heftzählung: 3

Seiten: 445 – 453

ISSN: 0302-766x

Sprache

Englisch

Verweise

DOI: 10.1007/s00441-012-1482-9

Pubmed: 22968961

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Despite the discovery of the widely expressed CLCA (chloride channel regulators, calcium-activated) proteins more than 15 years ago, their seemingly diverse functions are still poorly understood. With the recent generation of porcine animal models for cystic fibrosis (CF), members of the porcine CLCA family are becoming of interest as possible modulators of the disease in the pig. Here, we characterize pCLCA2, the porcine ortholog of the human hCLCA2 and the murine mCLCA5, which are the only CLCA members expressed in the skin. Immunohistochemical studies with a specific antibody against pCLCA2 have revealed a highly restricted pCLCA2 protein expression in the skin. The protein is strictly co-localized with filaggrin and trichohyalin in the granular layer of the epidermis and the inner root sheath of the hair follicles, respectively. No differences have been observed between the expression patterns of wild-type pigs and CF transmembrane conductance regulator(-/-) pigs. We speculate that pCLCA2 plays an as yet undefined role in the structural integrity of the skin or, possibly, in specialized functions of the epidermis, including barrier or defense mechanisms.