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    miR-Q: qRT-PCR for Expression Profiling of Small RNAs Such as MicroRNAs (2009)

    Art
    Vortrag
    Autor
    Sharbati, S
    Kongress
    Quantitative PCR
    San Diego/USA, 16. – 17.03.2009
    Quelle
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://www.healthtech.com/Conferences_Overview.aspx?id=83052&c=540
    Kontakt
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
    Tel.+49 30 838 62225 Fax.+49 30 838-62584
    email:biochemie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The miR-Q approach is a highly sensitive quantitative reverse transcription PCR (qRT-PCR) for expression analysis of small RNAs such as microRNA molecules. It is based on SYBR Green detection without requiring fluorochromic probes or Locked Nucleic Acid (LNA)-modified oligonucleotides. It shows a high dynamic range of six to eight orders of magnitude comprising a sensitivity of up to 0.2 fM microRNA, which corresponds to single copies per cell. There is nearly no cross reaction among closely-related microRNA family members, which points to the high specificity of the assays.