Publikationsdatenbank

miR-Q: qRT-PCR for Expression Profiling of Small RNAs Such as MicroRNAs (2009)

Art

Vortrag

Autor

Sharbati, S

Kongress

Quantitative PCR

San Diego/USA, 16. – 17.03.2009

Quelle

Sprache

Englisch

Verweise

URL (Volltext): http://www.healthtech.com/Conferences_Overview.aspx?id=83052&c=540

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The miR-Q approach is a highly sensitive quantitative reverse transcription PCR (qRT-PCR) for expression analysis of small RNAs such as microRNA molecules. It is based on SYBR Green detection without requiring fluorochromic probes or Locked Nucleic Acid (LNA)-modified oligonucleotides. It shows a high dynamic range of six to eight orders of magnitude comprising a sensitivity of up to 0.2 fM microRNA, which corresponds to single copies per cell. There is nearly no cross reaction among closely-related microRNA family members, which points to the high specificity of the assays.