Fachbereich Veterinärmedizin



    Varicella-zoster virus-induced apoptosis in MeWo cells is accompanied by down-regulation of Bcl-2 expression (2010)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Brazeau, Elizabeth
    Mahalingam, Ravi
    Gilden, Don
    Wellish, Mary
    Kaufer, Benedikt B
    Osterrieder, Nikolaus
    Pugazhenthi, Subbiah
    Journal of neurovirology; 16(2) — S. 133–140
    ISSN: 1355-0284
    DOI: 10.3109/13550281003682547
    Pubmed: 20345323
    Institut für Virologie

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    Abstract / Zusammenfassung

    Varicella-zoster virus infects multiple human and monkey cells in culture. The mode of cell death appears to be autophagy or apoptosis. Analysis of VZV-infected human melanoma (MeWo) cells revealed that Bcl-2 mRNA and protein levels were decreased significantly 64 and 72 hpi (hours post infection), accompanied by the release of cytochrome c from mitochondria into the cytoplasm. Western blot analysis of virus-infected cells revealed activation of caspase-8, a marker for the extrinsic pathway of apoptosis, and caspase-9, a marker for the intrinsic pathway of apoptosis at 64 and 72 hpi. Significant increases in the levels of cleaved caspase-3 and cleaved poly (ADP) ribose polymerase (PARP) were also seen at the height of cytopathic effect. Thus VZV induces apoptosis in MeWo cells in which Bcl-2 is down-regulated. Future studies will determine differences in the cascade of apoptotic events in non-neuronal cells compared to neurons that allow VZV to become latent.