Fachbereich Veterinärmedizin



    Lipid composition of virosomes modulates their fusion efficiency with cryopreserved bull sperm cells (2001)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Markgraf, K
    Müller, K
    Ponimaskin, E G
    Rudolph, M
    Schmidt, M F
    Herrmann, A
    Cloning; 3(1) — S. 11–21
    ISSN: 1520-4553
    Pubmed: 11918838
    Institut für Immunologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51834

    Abstract / Zusammenfassung

    Virosomes derived from different fusogenic enveloped viruses have been generated for potential application in gene targeting to sperm cells. Comparative characterization of reconstitution products revealed that virosomes derived from influenza viruses are superior to those generated from Sendai viruses, with respect to the fusion rates with cryopreserved bull sperm cells and to sperm cell vitality after fusion. Modulation of the lipid composition during virosome reconstitution affects fusion sites on target sperms and allows optimization of the fusion rate and sperm cell vitality. A fluorescence-based microscopic fusion assay combined with a vital cell stain revealed that about 90% of sperm cells fused with influenza virosomes containing exogenous cholesterol, sphingomyelin, phosphatidylcholine, and phosphatidylethanolamine. About 85% of the fused sperm cells remained vital. Such optimized influenza-derived virosomes provide the basis for ongoing experiments, which aim at eventually generating biologically active transgenic sperms.