Publikationsdatenbank

Lipid composition of virosomes modulates their fusion efficiency with cryopreserved bull sperm cells (2001)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Markgraf, K

Müller, K

Ponimaskin, E G

Rudolph, M

Schmidt, M F

Herrmann, A

Quelle

Cloning

Bandzählung: 3

Heftzählung: 1

Seiten: 11 – 21

ISSN: 1520-4553

Sprache

Englisch

Verweise

Pubmed: 11918838

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Virosomes derived from different fusogenic enveloped viruses have been generated for potential application in gene targeting to sperm cells. Comparative characterization of reconstitution products revealed that virosomes derived from influenza viruses are superior to those generated from Sendai viruses, with respect to the fusion rates with cryopreserved bull sperm cells and to sperm cell vitality after fusion. Modulation of the lipid composition during virosome reconstitution affects fusion sites on target sperms and allows optimization of the fusion rate and sperm cell vitality. A fluorescence-based microscopic fusion assay combined with a vital cell stain revealed that about 90% of sperm cells fused with influenza virosomes containing exogenous cholesterol, sphingomyelin, phosphatidylcholine, and phosphatidylethanolamine. About 85% of the fused sperm cells remained vital. Such optimized influenza-derived virosomes provide the basis for ongoing experiments, which aim at eventually generating biologically active transgenic sperms.