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    Molecular cloning, expression and characterization of the Canis familiaris interleukin-4 (2001)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Wondimu, A
    Veit, M
    Kohn, B
    Kaul, S
    Hoffmann, A
    Brunnberg, L
    Schmidt, M F
    Quelle
    Cytokine; 16(3) — S. 88–92
    ISSN: 1043-4666
    Sprache
    Englisch
    Verweise
    Pubmed: 11741347
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    Abstract / Zusammenfassung

    Interleukin 4 (IL-4) is an important regulatory cytokine produced by activated T lymphocytes and mast cells, and regulates the growth and differentiation of cells such as B and T lymphocytes. The rapid amplification of cDNA ends (RACE) was used to clone the canine IL-4 gene. It was expressed in mammalian cells and Escherichia coli. Monoclonal antibodies were raised to rcIL-4 for use in an enzyme-linked immunosorbent assay (ELISA). This will facilitate studies on the role of cIL-4 in inflammatory diseases, particularly rheumatoid arthritis.