Publikationsdatenbank

Molecular cloning, expression and characterization of the Canis familiaris interleukin-4 (2001)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Wondimu, A

Veit, M

Kohn, B

Kaul, S

Hoffmann, A

Brunnberg, L

Schmidt, M F

Quelle

Cytokine

Bandzählung: 16

Heftzählung: 3

Seiten: 88 – 92

ISSN: 1043-4666

Sprache

Englisch

Verweise

Pubmed: 11741347

Kontakt

Klein- und Heimtierklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62422
kleintierklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Interleukin 4 (IL-4) is an important regulatory cytokine produced by activated T lymphocytes and mast cells, and regulates the growth and differentiation of cells such as B and T lymphocytes. The rapid amplification of cDNA ends (RACE) was used to clone the canine IL-4 gene. It was expressed in mammalian cells and Escherichia coli. Monoclonal antibodies were raised to rcIL-4 for use in an enzyme-linked immunosorbent assay (ELISA). This will facilitate studies on the role of cIL-4 in inflammatory diseases, particularly rheumatoid arthritis.