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    The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues (2004)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autor
    Veit, Michael
    Quelle
    The Biochemical journal; 384(2) — S. 233–237
    ISSN: 0264-6021
    Sprache
    Englisch
    Verweise
    Pubmed: 15479160
    Kontakt
    Institut für Immunologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51834

    Abstract / Zusammenfassung

    The yeast SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) protein Ykt6 was shown to mediate palmitoylation of the fusion factor Vac8 in a reaction essential for the fusion of vacuoles. Here I present evidence that hYkt6 (human Ykt6) has self-palmitoylating activity. Incubation of recombinant hYkt6 with [3H]Pal-CoA ([3H]palmitoyl-CoA) leads to covalent attachment of palmitate to C-terminal cysteine residues. The N-terminal domain of human Ykt6 contains a Pal-CoA binding site and is required for the reaction.