Publikationsdatenbank

The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues (2004)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autor

Veit, Michael

Quelle

The Biochemical journal

Bandzählung: 384

Heftzählung: 2

Seiten: 233 – 237

ISSN: 0264-6021

Sprache

Englisch

Verweise

Pubmed: 15479160

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The yeast SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) protein Ykt6 was shown to mediate palmitoylation of the fusion factor Vac8 in a reaction essential for the fusion of vacuoles. Here I present evidence that hYkt6 (human Ykt6) has self-palmitoylating activity. Incubation of recombinant hYkt6 with [3H]Pal-CoA ([3H]palmitoyl-CoA) leads to covalent attachment of palmitate to C-terminal cysteine residues. The N-terminal domain of human Ykt6 contains a Pal-CoA binding site and is required for the reaction.