Publikationsdatenbank

The SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion (2004)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Dietrich, Lars E P

Gurezka, Rolf

Veit, Michael

Ungermann, Christian

Quelle

The EMBO journal

Bandzählung: 23

Heftzählung: 1

Seiten: 45 – 53

ISSN: 0261-4189

Sprache

Englisch

Verweise

Pubmed: 14685280

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The NSF homolog Sec18 initiates fusion of yeast vacuoles by disassembling cis-SNARE complexes during priming. Sec18 is also required for palmitoylation of the fusion factor Vac8, although the acylation machinery has not been identified. Here we show that the SNARE Ykt6 mediates Vac8 palmitoylation and acts during a novel subreaction of vacuole fusion. This subreaction is controlled by a Sec17-independent function of Sec18. Our data indicate that Ykt6 presents Pal-CoA via its N-terminal longin domain to Vac8, while transfer to Vac8's SH4 domain occurs spontaneously and not enzymatically. The conservation of Ykt6 and its localization to several organelles suggest that its acyltransferase activity may also be required in other intracellular fusion events.