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    Complex porcine model of atherosclerosis: Induction of early coronary lesions after long-term hyperlipidemai without sustained hyperglycemia (2009)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Artinger, S*
    Deiner, C*
    Loddenkemper, C.
    Schwimmbeck, P. L.
    Schultheiss, H-P
    Pels, K.
    Quelle
    The Canadian Journal of Cardiology; 25(4) — S. e109–e114
    ISSN: 0828-282x
    Sprache
    Englisch
    Verweise
    Pubmed: 19340354
    Kontakt
    Institut für Veterinär-Physiologie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62600
    physiologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Background: The incidence of coronary artery disease (CAD) is still increasing in industrialized countries and it is even higher in diabetic patients. For experimental studies investigating the pathophysiology of CAD, the use of an animal model comparable to the pathologic situation in patients is crucial.
    Objective: To develop a model of advanced coronary atherosclerosis with induction of hyperlipidemia and hyperglycemia in domestic pigs.
    Methods: Six pigs were fed a standard pig chow (controls), two were fed a 2 % cholesterol and 17 % coconut fat diet (Chol group), and two pigs received a 4 % cholesterol and 17 % coconut fat diet combined with Streptozotocin (STZ) injections to induce diabetes (High Chol+STZ group). Serum lipid and plasma glucose values were analyzed, and histochemical staining for morphometric analysis and immunohistochemistry were performed.
    Results: Pigs on hyperlipidemic diet had elevated mean (± SD) serum lipid levels (total cholesterol: 5.05±1,45 mmol/L [Chol] and 5.03±2,41 mmol/L [High Chol+STZ] versus 2.09±0,23 mmol/L [controls]). Histopathologic evaluation revealed an initial stage of coronary atherosclerosis. None of the ST-treated pigs showed a sustained elevation of plasma glucose (mean glucose before STZ injection was 5.11±0.94 mmol/L and thereafter was 6.03±2.39 mmol/L) or a decline in pancreatic beta cells.
    Conclusions:
    The current data suggest that the domestic porcine model is not suitable to create severe CAD using an atherogenic diet in combination with STZ injections for experimental interventional vascular research. This may be due to different STZ sensitivities among species. However, hyperlipidemia induced early pathological lesions in coronary arteries resembling initial stages of atherosclerosis without severe luminal narrowing.