Publikationsdatenbank

Age-dependent response of the acid-base parameters (Henderson-Hasselbalch, Stewart) in healthy calves with experimentally induced metabolic acidosis (2009)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Elkhair, N. M.

Siegling-Vlitakis, C.

Radtke, E.

Willing, A.

Hartmann, H.

Quelle

Berliner und Münchener tierärztliche Wochenschrift : BMTW

Bandzählung: 122

Heftzählung: 1-2

Seiten: 63 – 69

ISSN: 1439-0299

Sprache

Englisch

Verweise

Pubmed: 19226937

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The intensity of the response to acid-base parameters in relation to the age after a defined acid load was studied in calves.
32 clinically healthy calves (age: 4-104days) were infused with 5M NH4Cl solution (dose: 1.0ml/kg) through a permanent intravenous catheter. Before (0 hrs) and after starting the infusion (2, 4, 6, 8 and 24hrs) venous blood samples were collected for the determination of the various acid-base parameters. The intensity of the response of the acid-base parameters was estimated by using the ?area under curve (AUC)? procedure.
By 2-6hrs after the infusion of the NH4Cl solution, the Henderson-Hasselbalch parameters decreased significantly (pH,[HCO3-]) as did Stewart?s variables ([Strong ion difference=SID3],[Acid total=Atot or A-]). A transient moderate hyperchloraemic acidosis with a slight hypoproteinaemic alkalosis was observed in all calves in association with a respiratory compensation (PCO2). The younger calves (1st-3rdweek) showed a similar pattern of response to the same dose per kg0.75 acid load with significantly greater acid-base parameters response (higher AUC values) than the older animals. The calculated pH was determined by using the three Stewart variables PvCO2, serum-[SID3] and serum-[Atot]. The mean difference was
-0.03 to -0.09 compared with the measured pH (7.32-7.40).
Conclusion: The Stewart model appears to be more successful in providing a comprehensive evaluation of acid-base status compared with the traditional Henderson-Hasselbalch model. The younger calves during the first few weeks of life reacted more sensitively to an equal acidotic condition than the older animals.