Fachbereich Veterinärmedizin



    Rapid testing leads to the underestimation of the scrapie prevalence in an affected sheep and goat flock (2007)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Reckzeh, Claudia
    Hoffmann, Christine
    Buschmann, Anne
    Buda, Silke
    Budras, Klaus-Dieter
    Reckling, Karl-Friedrich
    Bellmann, Steffi
    Knobloch, Hartmut
    Erhardt, Georg
    Fries, Reinhard
    Groschup, Martin H
    Veterinary Microbiology; 123(4) — S. 320–327
    ISSN: 0378-1135
    Pubmed: 17521831
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
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    Abstract / Zusammenfassung

    To obtain a more detailed understanding of the prevalence of classical scrapie infections in a heavily affected German sheep flock (composed of 603 sheep and 6 goats), we analysed 169 sheep and 6 goats that carried the genotypes susceptible to the disease and that were therefore culled following discovery of the index case. The initial tests were performed using the Biorad TeSeE ELISA and reactive results were verified by official confirmatory methods (OIE-immunoblot and/or immunohistochemistry (IHC)) to demonstrate the deposition of scrapie-associated PrP(Sc) in the brain stem (obex). This approach led to the discovery of 40 additional subclinically scrapie-infected sheep. Furthermore, peripheral lymphatic and nervous tissue samples of the 129 sheep and 6 goats with a negative CNS result were examined by IHC in order to identify any preclinical infections which had not already spread to the central nervous system (CNS). Using this approach we found 13 additional sheep with PrP(Sc) depositions in the gut-associated lymph nodes (GALT) as well as in the enteric nervous system. Moreover, in most of these cases PrP(Sc) was also deposited in the spleen and in the retropharyngeal and superficial cervical lymph nodes. Taken together, these results show a 30.3% infection prevalence in this scrapie-affected flock. Almost 7.4% of the infected animals harboured PrP(Sc) exclusively in the peripheral lymphatic and nervous tissue and were therefore missed by the currently used testing strategy.