Fachbereich Veterinärmedizin



    Avian influenza H5N1 infections in vaccinated commercial poultry and backyard birds in Egypt (2010)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Hafez, H. M.
    Arafa, A.
    Abdelwhab, E. M.
    Selim, A.
    Khoulosy, S. G.
    Hassan, M. K.
    Aly, M. M.
    Poultry Science; 89(8) — S. 1609–1613
    ISSN: 0032-5791
    Pubmed: 20634514
    Institut für Geflügelkrankheiten

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    Abstract / Zusammenfassung

    In this paper, we describe results from a high-pathogenic H5N1 avian influenza virus (AIV) surveillance program in previously H5-vaccinated commercial and family-backyard poultry flocks that was conducted from 2007 to 2008 by the Egyptian National Laboratory for Veterinary Quality Control on Poultry Production. The real-time reverse transcription PCR assay was used to detect the influenza A virus matrix gene and detection of the H5 and N1 subtypes was accomplished using a commercially available kit real-time reverse transcription PCR assay. The virus was detected in 35/3,610 (0.97%) and 27/8,682 (0.31%) of examined commercial poultry farms and 246/816 (30%) and 89/1,723 (5.2%) of backyard flocks in 2007 and 2008, respectively. Positive flocks were identified throughout the year, with the highest frequencies occurring during the winter months. Anti-H5 serum antibody titers in selected commercial poultry ranged from <2 (negative) to 9.6 log(2) when determined in the hemagglutination inhibition test using a H5 AIV antigen. In conclusion, despite the nationwide vaccination strategy of poultry in Egypt to combat H5N1 AIV, continuous circulation of the virus in vaccinated commercial and backyard poultry was reported and the efficacy of the vaccination using a challenge model with the current circulating field virus should be revised.