The objective is focused on the impact of luminal factors on intestinal microRNA response. Feed additives but also pathogens will taken into consideration. The impact of intestinal miRNAs on corresponding targets and their role on optimal pathogen deference will be analysed using a primary porcine intestinal epithel cell culture model. The resulting in vitro data will be verified within the later phase of the project using an in vitro model. The approach will contribute to a better understanding of regulation factors, and now local aspects may be provided for novel therapeutic strategies.