The main aim of the project is a generation of the HVT vectored recombinant vaccines. This project (i) establishes new non-essential sites within HVT genome and, (ii) uses these non-essential sites as insertion of selected protective antigens from IBDV, NDV, TRT and ILTV. In the first phase BAC clone(s) of HVT will be constructed. The BAC clones will be utilized to confirm non-essential sites and to evaluate/select promoter(s) and to assess expression of foreign genes. Once above is accomplished, new HVT construct will be generated using standard recombination methods.